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Order the steps in gel electrophoresis

  • DNA is treated with a chemical which causes the molecule to fluoresce
    1st
  • Fragments of DNA are loaded into wells (in agar gel)
    2nd
  • The DNA is subjected to a electric current, with the negative end closest to the starting point of the DNA
    3rd
  • DNA fragments move toward the positively charged electrode
    4th
  • Smaller fragments are more easily able to move through the pores in the agar gel and thus move a larger distance in the same time
    5th
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